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KMID : 0380020070220050313
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 5 p.313 ~ p.317
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Effect of Pluronic F-68 on the Post-thaw Growth of Cryopreserved Transgenic Nicotiana tabacum Cells
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Jeon Su-Hwan
Lee Kyoung-Hoon
Kwon Joon-Young
Kim Dong-Il
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Abstract
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To enhance the growth of cryopreserved cells of transgenic Nicotiana tabacum, Pluronic F-68 was supplemented in a recovery medium during post-thaw period. As cryoprotective agents, 1 M sucrose, 0.5 M glycerol and 0.5 M dimethyl sulfoxide (DMSO) were added before freezing steps. The post-thaw growth of the cells was improved with Pluronic F-68, ranged from 0.1 to 10 g/L. The interactions of Pluronic F-68 with the cells were confirmed by the changes of hydrophobicity or permeability of the cells. Pluronic F-68 did not show any effect on the activity of -glucuronidase (GUS) in all treatments. Therefore, the addition of Pluronic F-68 in a recovery medium was found to be beneficial to enhance the post-thaw growth of cryopreserved transgenic tobacco cells without affecting the production of recombinant protein.
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KEYWORD
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Pluronic F-68, cryopreservation, Nicotiana tabacum, post-thaw growth, transgenic plant cell cultures
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